1/14/2024 0 Comments Serial dilution![]() To aspirate the desired volume, the pipette should be dipped just below the liquid surface. The pipette should be held at a consistent angle, maximum 20° during the dilution process. When the liquid is drawn, the inclination angle of the pipette also influences the liquid volume in the tip. Errors could be introduced into the subsequent dilution steps if pre-wetting is not done, which results in a smaller delivery volume in the initial dispenses. This process equilibrates the temperature differences between tip and liquid, and humidifies the dead air space inside the tip and pipette. ![]() This can be done by aspirating and dispensing the full volume two to three times. Pre-wetting of pipette tips should be performed prior to preparing the assay. To ensure that pipettes are employed in an optimal and reproducible way, good pipetting practice elucidates guidelines to cover the basic techniques. In addition, the article outlines the features an automated pipetting system must have to ensure high reproducibility of serial dilutions and introduces the ASSIST, a custom-designed product for this task. This article also discusses different mixing parameters to help users achieve a homogenous sample, as mixing is a vital component of dilution protocols.Īutomating the process provides many benefits as it maximizes consistency of dilution assays. This article focuses on a range of good pipetting techniques, which when followed provide the best way for enhancing and maintaining the reproducibility of serial dilutions. In order to improve reproducibility and accuracy, good pipetting practice should be observed. The human influence impacts most on pipetting results, assuming that pipettes are properly maintained and calibrated. Since pipetting serial dilutions is a time-consuming process and is consequently prone to errors, particularly in 384 well plates, pipetting serial dilutions reproducibly can be very difficult. Sponsored Content by INTEGRA Biosciences Mar 14 2017
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